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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
Chip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simple chip enzymatic chromatin ip kit  (Cell Signaling Technology Inc)
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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
Simple Chip Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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<t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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YAP directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) ChIP-qPCR analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.

Journal: Redox Biology

Article Title: NMRK2–YAP–NADK axis preserves redox protection against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2026.104100

Figure Lengend Snippet: YAP directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) ChIP-qPCR analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.

Article Snippet: ChIP assays were performed using a commercial ChIP kit (CST 9005S) to assess the binding of YAP to the NADK promoter.

Techniques: Binding Assay, Luciferase, Construct, ChIP-qPCR, Over Expression, Activity Assay, Activation Assay, Chromatin Immunoprecipitation, Negative Control